Differential Media:
Glucose Fermentation Broth and O/F Medium – Page 2

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The following examples show the behavior of four organisms in both Glucose Fermentation Broth and Glucose O/F Medium. The organisms are gram-negative except Staphylococcus epidermidis which grows weakly in Glucose O/F Medium.

"EC" = Escherichia coli
"PF" = Pseudomonas fluorescens
"AF" = Alcaligenes faecalis
"SE" = Staphylococcus epidermidis
"1" = Glucose O/F Medium
"2" = same with mineral oil overlay
"3" = Glucose Fermentation Broth
  Escherichia
coli
Pseudomonas
fluorescens
  Alcaligenes 
faecalis
Staphylococcus
epidermidis
Organism ferments glucose (and can grow anaerobically as a result) +
(Also produces H2 and CO2)
+
Acidic reaction from fermentation seen throughout both media +
(Note gas in Durham tube)
+
(Weak growth in Glucose O/F Medium)
Organism respires glucose
+ + +
Acidic reaction from respiration seen in open Glucose O/F Medium tube Can't tell due to acid from fermentation permeating medium +
(Alkaline reaction from peptone becomes apparent)
Can't tell due to acid from fermentation permeating medium
Reaction recorded for Glucose O/F Medium F O (weak F)

Page last modified on 9/18/02 at 5:15 PM, CDT.
John Lindquist:  new homepage, complete site outline.
Department of Bacteriology, U.W.-Madison

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